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Lin28 Monoclonal Antibody PR-3D1

Cytoplasmic staining with clone PR-3D1
Lin28 Monoclonal Antibody PR-3D1
Prod. No.
S2110-100UG
$300.00

Background

Human Lin28, encoded by the LIN28 gene, is a cytoplasmic mRNA-binding protein with a cold shock domain and two CHCC-type zinc fingers. It has been shown to enhance translation of IGF-2 mRNA and to block processing of let-7 microRNA. Lin28 is a marker for undifferentiated embryonic stem cells, and Lin28 expression has been used to enhance the generation of induced pluripotent stem (iPS) cells.




Clone: PR-3D1
Reacts with: Human, Mouse*
Tested Applications: WB, ELISA, Dot, Flow, IIF
Host: Mouse
Isotype: IgG1, κ
Size: 100 µg
Immunogen: Synthetic peptide derived from human Lin28 (within 50 amino acids of N-terminus) conjugated to ovalbumin carrier protein.

Purification: Protein G chromatography
Formulation: 1 mg/ml in PBS; Preservative: 0.09% NaN3
Storage:Store at 4ºC (<3 months) or -20ºC. Avoid multiple freeze-thaws.

Recommended Uses:
Western Blot 1:500
ELISA (detect) 1:1000
Dot blot (detect) 1:500
Note: Optimal concentrations should be empirically determined.
*Other species not tested

Citations
Meirelles, K., Benedict, L.A., Dombkowski, D. et al. (2012). "Human ovarian cancer stem/progenitor cells are stimulated by doxorubicin but inhibited by Mullerian inhibiting substance" Proc. Natl. Acad. Sci. USA 109:2358-2363. Free full text in PMC


Target Alternative Names: Protein lin-28 homolog A, Lin-28A, Zinc finger CCHC domain-containing protein
Target Size:209 AA; 22.7 kDa
Target Database Links:
RefSeq Protein NP_078950
RefSeq Gene NM_024674
UniProt Q9H9Z2

S2110-100UG

S2110

Support Files:

Certificate of Analysis: