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Nanog Monoclonal Antibody PR-22F3

Nanog Monoclonal Antibody PR-22F3
Prod. No.


Nanog is a transcription factor that is critical for maintaining embryonic stem (ES) cell pluripotency and preventing differentiation to germ layers and mature cell lineages. The Nanog protein contains a conserved DNA-binding homeobox domain (AA 98-154). When transfected together with other key pluripotent factors such as OCT4 (Pou5F1) and SOX2, Nanog has been used to enhance the reprogramming of somatic cells into induced pluripotent stem (iPS) cells.

Clone: PR-22F3
Reacts with: Human*
Tested Applications: Flow, ELISA
Host: Mouse
Isotype: IgG3, κ
Size: 100 µg
Immunogen: Recombinant full-length human Nanog expressed in E. coli and refolded to soluble form.
Purification: Protein G chromatography
Formulation: 0.983 mg/ml in PBS
Storage: Store at 4ºC (<3 months) or -20ºC. Avoid multiple freeze-thaws.

Recommended Uses:
ELISA (detect) 1:1000
Flow Cytometry 1µg/105 cells
Note: Optimal concentrations should be empirically determined.
*Other species not tested

Target Alternative Names: Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog
Target Database Links:
RefSeq Protein NP_079141.1
RefSeq Gene NM_024865.1
SwissProt Q9H9S0



Support Files:

Certificate of Analysis: