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POU5F1 (Oct4) Monoclonal Antibody PR-2H9

POU5F1 (Oct4) Monoclonal Antibody PR-2H9
Prod. No.


POU5F1, commonly known as Octamer-binding protein 4 (Oct4), is a POU transcription factor family member that is critical for maintaining embryonic stem (ES) cell pluripotency and preventing differentiation to germ layers and mature cell lineages. For this reason, POU5F1 expression is frequently used as a marker for undifferentiated stem cells. It contains a POU-specific domain and a homeobox DNA-binding domain. When transfected together with other key pluripotent transcription factors, POU5F1 has been used to reprogram somatic cells into induced pluripotent stem (iPS) cells.

Clone: PR-2H9
Reacts with: Human*
Tested Applications: WB, ELISA, Dot
Host: Mouse
IsotypeIgG3, κ

Size: 100 µg
Immunogen: Recombinant human POU5F1 (Oct4) fragment (AA 1-212) expressed in E. coli and refolded to soluble form.
Purification: Protein G chromatography
Formulation: 1 mg/ml in PBS
Preservative: 0.09% NaN3
Storage: Store at 4ºC (<3 months) or -20ºC.
Avoid multiple freeze-thaws.
Recommended Uses:
Western Blot 1:1000
ELISA (detect) 1:200-1:1000
Note: Optimal concentrations should be empirically determined.

Target Alternative Names:
POU domain, class 5, transcription factor 1; Octamer-binding protein 4; Oct-4; Oct-3; Oct-3/4; OTF-3

Target Size: 360 AA; 38.6 kDa

Target Database Links:
RefSeq Protein NP_002692.2
RefSeq Gene NM_002701.4
UniProt Q01860



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